Figure 4. T lymphocyte proliferation assay.

7 days after the second immunization(day 21), splenic lymphocytes from mice(groups containing CpG) were cultured with 10 μg/ml of MUC1₁₂ peptide. After 72 h, cell viability was measured by MTT assay A, B. Statistics relating to percentage of CD8⁺T cells among CD3⁺ T cells after simulation detected by flow cytometry. Each data point represents an individual mouse, and the horizontal lines indicate the mean± SD for the group of mice(r = 3, n = 3).