Figure 6. The degree of cytotoxicity of MUC1 peptide-stimulated splenic lymphocytes (effector cells) in mice from three groups immunized with the formulation containing CpG and normal control mice on MUC1⁺ B16 cells.

A. and G418-resistant B16 cells B. (target cells) depends on the ratio of splenic lymphocytes to MUC1⁺ B16 cells (Effector:Target). MUC1⁺ B16 cells were labeled with CFSE and co-cultured with MUC1 peptide-stimulated splenic lymphocytes at the ratios indicated above. When the ratios was “0”, it means “only target cells”. Cells were co-cultured for 4 h at 37°C. At the end of the experiment, dead cells were labeled with 7-AAD. The percentage of MUC1⁺ B16 cell lysis at different Effector:Target ratios was analyzed by a flow cytometer (r = 3, n = 3).