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. 2015 Oct 7;6(33):34592–34605. doi: 10.18632/oncotarget.5423

Figure 2. PINCH1 and ILKAP knockdown modulated clonogenic survival of glioblastoma cells.

Figure 2

A. Immunofluorescence staining of indicated proteins in A172 glioblastoma cells 24 h after plating. B. Immunofluorescence staining of A172 cells transiently transfected with an ILKAP-GFP construct. C. Western blot of whole protein lysates from siRNA-mediated A172, U87MG, LN229 and U138MG knockdown cell cultures and detection of PINCH1, ILK and ILKAP. β-Actin served as loading control. D. Clonogenic survival of PINCH1, ILK or ILKAP knockdown cell cultures. Data are mean ± SD (n = 3; t-test; *P < 0.05, **P < 0.01).