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. 2015 Oct 7;6(33):34592–34605. doi: 10.18632/oncotarget.5423

Figure 5. Expression of wildtype p53 was critical for the radiosensitizing effect upon ILKAP knockdown.

Figure 5

A. Protein expression and B. densitometric evaluation of ILKAP, p53, phospho-p53 (pp53) and p21 in p53 wildtype A172 and U87MG glioblastoma cells 15 and 60 minutes after irradiation with 6 Gy. Unirradiated cells were used as control. C. Basal and radiation survival of U87MG cells after p53 or ILKAP single knockdown and p53/ILKAP double knockdown. Efficacy of siRNA transfection was confirmed by Western blotting. Non-specific siRNA served as control (Co siRNA). Western blot for p53 D. and ILKAP knockdown E. and surviving fraction of F. unirradiated and G. irradiated U87MG stably transfected with shRNA directed against p53 (shp53). shRNA directed against luciferase (shLuc) was used as control. ILKAP knockdown was performed using siRNA (Co, non-specific siRNA control). Data are mean ± SD (n = 3; t-test; *P < 0.05, **P < 0.01).