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. 2015 Sep 22;6(33):34658–34668. doi: 10.18632/oncotarget.5778

Figure 5. Increase of cytoplasmic RMRP level following Wnt3a treatment.

Figure 5

A. Quantitation of Fluorescence In Situ Hybridization (FISH) data as shown in Figure S4B. HeLa cells were stained with RMRP probe and the percentage of cells with cytoplasmic RMRP was counted from the stained cells (n = 65). B. Cellular fractionation analysis of RMRP. Nuclear and cytoplasmic levels of RMRP from the fractionated HeLa cells following Wnt3a treatment. A graph represents densitometer scanning of above gel. C. PCR analysis of mitochondrial DNA (mtDNA). Mitochondrial fractions were prepared and mtDNA was purified. Mitochondrial 16S rRNA gene was detected to measure the amount of mtDNA. Nuclear DNA (nDNA) contamination was also monitored by GAPDH and β-catenin genes. Wnt3a treatment for 3 hrs was executed in HeLa cells.