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. 2015 Sep 22;6(33):34691–34703. doi: 10.18632/oncotarget.5787

Figure 3. H19 inhibition attenuates hEC cell proliferation.

Figure 3

A. Down-regulation of H19 in NCCIT cells induces a reduction of cell proliferation as assessed by the BrdU incorporation assay (n = 3). B. FACS analysis of the cell cycle of the transduced NCCIT/shH19 cells reveals a decrease in cells residing in S phase, and a shift from S phase to G0/G1 phase (n = 3). C. A decrease in cell numbers following H19 down-regulation in NCCIT/shH19 as revealed by Methylene blue assay. Data are represented as mean ± SD; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.