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. 2015 Sep 22;6(33):34691–34703. doi: 10.18632/oncotarget.5787

Figure 4. H19 knockdown decreases hEC cell-matrix attachment and decreases hEC cell-cell interactions.

Figure 4

A. Down-regulation of H19 expression in NCCIT cells causes a marked change in cell morphology with failure of the cells to attach to the matrix. B. H19 inhibition in NCCIT induces up-regulation of E-Cadherin and down-regulation of N-Cadherin expression while Vimentin and Keratin-18 expression levels remain unchanged (qPCR; n = 4). C. Up-regulation of E-Cadherin protein levels in NCCIT/shH19 cells as detected by Western blot. D. NCCIT/shH19 cells exhibit a decreased organization of F-actin: Immunohistofluorescence staining for F-Actin (Green) and nuclear counterstaining with Dapi (Blue).