Skip to main content
. 2015 Sep 25;6(33):34704–34717. doi: 10.18632/oncotarget.5474

Figure 1. 12-O-tetradecanoylphorbol-13-acetate (TPA) activates the re-expression of COX-2 gene.

Figure 1

A. mRNAs from the human lung cancer cell lines H719, H460, H23 and A549 were extracted to detect COX-2 expression by RT-PCR. GAPDH was used as a loading control. P indicates a positive control, and N indicates a negative control. B. H719 cells were treated with TPA for 6 hrs at different doses (0, 15, 50 and 100 ng/mL), or for different times (0, 2, 4 and 6 hrs) at 100 ng/mL. RT-PCR was performed to detect COX-2 mRNA levels after treatment. GAPDH was used as a loading control. C. H460 cells were treated with TPA for 6 hrs at different doses (0, 15, 50 and 100 ng/mL), or for different times (0, 2, 4 and 6 hrs) at 100 ng/mL. RT-PCR was performed to detect the COX-2 mRNA levels after treatment. GAPDH was used as a loading control. D. H719 and H460 cells were treated with TPA for different times (0, 2, 4 and 6 hrs) at 100 ng/mL, and proteins were extracted to detect the expression of COX-2 at the protein level by Western blotting. β-actin is shown as a loading control.