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. 2015 Sep 25;6(33):34704–34717. doi: 10.18632/oncotarget.5474

Figure 6. c-Fos recruits KDM2A to the COX-2 promoter.

Figure 6

A. H719 cells were transfected with KDM2A siRNA or non-specific siRNA (NS) for 48 hrs, followed by Western blotting to assess the efficiency of the RNAi. B. H719 cells were transfected with KDM2A siRNA or non-specific siRNA (NS) for 48 hrs and treated with TPA at 100 ng/mL for 2 hrs. A ChIP assay was performed to detect the binding activity of c-Fos around the COX-2 promoter. The bands containing anti-IgG served as negative controls. C. H719 cells were transfected with c-Fos siRNA or non-specific siRNA (NS) for 48 hrs and then treated with TPA for 4 hrs at 100 ng/mL. A ChIP assay was performed to detect the binding activity of KDM2A around the COX-2 promoter (the upper panel). ChIP-qPCR assay was used to detect the change in binding activity of KDM2A around the COX-2 promoter after 4 hrs of TPA treatment (the lower panel). *, p < 0.05. D. A schematic showing a possible mechanism by which TPA induces the methylated COX-2 gene re-expression. All symbols are defined in the diagram.