Skip to main content
. 2015 Sep 28;6(33):34788–34799. doi: 10.18632/oncotarget.5858

Figure 6. Hippo-YAP pathway caused by PAR1 activation promotes EMT, and ensures the preservation of cancer stem-like cell.

Figure 6

A. Even when Snail was inhibited by siRNA, PAR1 activation promoted dephophorylation of YAP1 and inhibited phosphorylation of Lats1. YAP1 and pLats1 (T1079) were detected by western blot assays. B. The cytosolic and nuclear expression levels of E-cadherin, fibronectin and Snail were detected by western blot assays. MKN45/PAR1 and MKN74 cells were pretreated with C3 for 5 h or YAP1 was knocked down by siRNA and then incubated with TFLLR-NH2 for indicated times. C. Immunofluorescence staining of anti-actin (green) shows the morphology of MKN45/PAR1 and MKN74 cells, which were pretreated with C3 for 5 h or YAP1 was knocked down by siRNA, were unchanged. D. FACS analysis of side population showed C3 treatment and knockdown of YAP1 efficiency blocked increase side population cells, even when MKN45/PAR1 and MKN74 cells were treated with TFLLR-NH2. E. MKN45/PAR1 and MKN74 cell cultures, which were pretreated with C3 for 5 h or YAP1 was knocked down by siRNA, filled in a scratch made with 200-μl pipette tip after 24 h similar to control cells, and MKN45/PAR1 and MKN74 cells treated with TFLLR-NH2 recovered more efficient than control cells. F. Invasion assay shows MKN45/PAR1 and MKN74 cells, which were pretreated with C3 for 5 h or YAP1 was knocked down by siRNA and then incubated with TFLLR-NH2, presented a significant decline in invasion potential relative to these cells treated with TFLLR-NH2 only. A560 nm of MKN45/mock and MKN74 cultured under a TFLLR-NH2 free condition as a baseline.