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. 2015 Oct 12;6(33):34846–34858. doi: 10.18632/oncotarget.5413

Figure 3. Washout procedure completely removes drug from the media.

Figure 3

A. Schematic of drug treatment experiment in (b). SEM and NALM-6 cells were continuously treated with DMSO or with 250 or 500 nM CX-5461 (CX) respectively. A portion of CX-5461 treated cells was harvested after 3 hours, washed twice and incubated in drug free media. After second wash, cells were suspended in drug free medium and spun again. Resulting supernatant was collected and added to drug naïve cells (S) while the cells pellet was suspended in fresh media (w/o). B. Cell viability was measured immediately after washout and at day 1 and 3 with trypan blue staining. Experiment was performed three times and mean +/− S.D. is plotted. C. SEM cells were continuously incubated in DMSO or CX-5461 and a portion was harvested after 3 hours followed by washing in drug free media as before. Cell viability was measured using trypan blue staining at day 0, 1, 2 and 3 after washout. Results are plotted as mean +/− S.D.