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. 2015 Oct 12;6(33):34846–34858. doi: 10.18632/oncotarget.5413

Figure 4. UCN-01 relieves CX-5461 induced G2/M phase arrest.

Figure 4

A. Cells were either treated with CX-5461 and UCN-01 alone or pretreated with 100 nM UCN-01 for 1 hour followed by 250 nM CX-5461 for 1 day. Cell-cycle distribution was determined by flow cytometry analysis of PI stained cells. UCN-01 completely removed G2/M block induced by CX-5461. One representative experiment out of three is shown. B. Cells were treated as in (a) and cell viability was measured at 55 hours post drug treatment using trypan blue staining. Combination treatment shows enhanced cytotoxicity compared to treatment with single agent. Experiment was repeated three times and mean +/− S.D. is plotted.