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. 2015 Oct 12;6(33):34859–34874. doi: 10.18632/oncotarget.5414

Figure 3. Knockdown of EMP3 inhibits the abilities of migration and invasion of HCC cells through down-regulation of MMP-9 and uPA.

Figure 3

A. Upper plot: the representative results of the in vitro migration and invasion assay. Lower plot: the relative abilities of migration and invasion of shEMP3 cells was compared to that of shLuc cells. B. The protein expressions of EMP3, MMP-9, and uPA were examined by immunoblotting. β-actin was an internal control. The relative amount of indicated protein was shown in the bottom plot from 3 independent experiments. C. Upper panel: the proteolytic activity was examined by zymography. Lower plot: the relative density of proteolytic band of MMP-9, MMP-2, and uPA from 3 independent experiments. D. The IF staining of EMP3 (green), MMP-9 (red), and uPA (red) and DAPI staining of nucleus (blue color) in each cell line. Scale bars = 50 μm. Data are presented as the mean ± SE of at least three independent experiments. **, p < 0.01.