Skip to main content
. 2015 Oct 12;6(33):34859–34874. doi: 10.18632/oncotarget.5414

Figure 4. Knockdown of EMP3 suppresses cell proliferation of HCC cells mainly through inactivation of PI3K/Akt pathway.

Figure 4

A. The expressions of indicated proteins were examined by immunoblotting. The relative amounts of indicated proteins in shEMP3 cells comparing to that in shLuc-infected cells were shown in the right plot. B. Cells were treated with or without 30 μM of PD98059 (a MEK inhibitor), or 30 μM of LY294002 (a PI3K inhibitor) for 2 h, or transfected with siRNA towards ERK or Akt, and then the cell growth was determined by MTT assay. C. Cells were treated with or without 30 μM of LY294002, or transfected with siRNA towards Akt. The clonogenic ability was examined by colony formation assay. The bottom plot was the quantitative results. D. Cell cycle was determined by flow cytometer. The percentage of cells distributed at G1 phase. The expressions of indicated proteins were examined by immunoblotting in E. Data are presented as the mean ± SE of at least three independent experiments. *, p < 0.05, **, p < 0.01 compared to the expression levels in shLuc cells; #, p < 0.01 compared to the expression levels in untreated shEMP3 cells.