Table 1. Characteristics and productions of VSVG epitope-incorporated Ads differed in the number of deleted amino acids (aa) in the HI-loop (0 aa-, 10 aa-, or 18 aa-deletion) and the number of surrounding 5 aa (GGSGS) linker sequence(s) (1, 2, or 3) on both end of the VSVG epitope.
Constructed vector | Deletion in HI-loop | Number of linker (GGSGS) | Viral production |
---|---|---|---|
RdB | no deletion | 0 | Yes |
RdB-1L-VSVG | no deletion | 1 | Yes |
RdB-2L-VSVG | no deletion | 2 | no |
RdB-3L-VSVG | no deletion | 3 | no |
RdB-d10-1L-VSVG | 10 deletion | 1 | no |
RdB-d10-2L-VSVG | 10 deletion | 2 | no |
RdB-d10-3L-VSVG | 10 deletion | 3 | no |
RdB-d18-1L-VSVG | 18 deletion | 1 | no |
RdB-d18-2L-VSVG | 18 deletion | 2 | no |
RdB-d18-3L-VSVG | 18 deletion | 3 | no |