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. 2015 Sep 30;6(33):34892–34909. doi: 10.18632/oncotarget.5408

Figure 4. Hph-1-gp70 enhances doxorubicin-induced apoptosis.

Figure 4

A. FM3A and B. MTT060562 cells were treated with 1 μM cont-gp70, Hph-1-GFP, or Hph-1-gp70 and 500 nM doxorubicin, and the apoptotic cell ratios were determined 24 h later with annexin V-staining. C. 3T3 cells transfected with HA-empty vector or HA-Mcm2 were treated with 1 μM Hph-1-GFP or Hph-1-gp70 and 500 nM doxorubicin, and the apoptotic cell ratios were determined 24 h later with annexin V-staining. D. FM3A and E. MTT060562 cells were treated with 1 μM Hph-1-GFP or Hph-1-gp70 for 24 h. The effects of treatment on the cell cycle profiles were analyzed with FACS. F. 3T3 cells transfected with HA-empty vector or HA-Mcm2 were treated with 1 μM Hph-1-GFP or Hph-1-gp70 for 24 h. The effects on the cell cycle profiles were analyzed with FACS. G. Representative western blots for DNA-PK, phospho-DNA-PK (pS2053), P53, phospho-P53, and cleaved caspase-3 in FM3A, MTT060562, and HA-Mcm2-transfected 3T3 cells. The cells were treated with 1 μM Hph-1-GFP or Hph-1-gp70 and 500 nM doxorubicin for 24 h. H. Mcm2 knockdown in FM3A and I. MTT060562 cells by using siRNA. Quantitative RT-PCR was performed to confirm the si-Mcm2-induced reduction in Mcm2 mRNA expression. J. FM3A and K. MTT060562 cells transduced with si-control or si-Mcm2 were treated with 1 μM Hph-1-GFP or Hph-1-gp70 and 500 nM of doxorubicin, and the apoptotic cell ratios were determined 24 h later with annexin V-staining. *P < 0.01, **P < 0.05 by two-tailed Student's t-test.