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. 2015 Oct 12;6(33):34953–34967. doi: 10.18632/oncotarget.5547

Figure 5. In vivo anti-lung cancer efficacy and acute toxicity of 6-OAP.

Figure 5

A. Nude mice subcutaneously inoculated with H1975 cells were treated with 6-OAP or vehicle for 24 days, and tumor volume was estimated every two days. B. Images of xenograft tumors obtained from the mice. C. Western blot assays using lysates of isolated tumors and indicated antibodies. D. A549-Luciferase cells were intravenously injected into SCID mice, and 1 week later the mice were randomized to receive vehicle (n = 6) or 6-OAP treatment (n = 12). The mice were detected by IVIS Spectrum. E. The relative luciferase intensity in the mice. F. Lung tissue sections of mice treated with 6-OAP or vehicle control were stained with hematoxylin-eosin and analyzed using a research microscope. Size bar, 100 μm. G. Life span of the mice. H. Western blot analysis using lysates of tumor samples isolated from the mice. I. Intravenous injection of 6-OAP at 225 to 600 mg/kg did not significantly affect the body weight of Kunming mice. J, K. The serum AST (J) and CR (K) levels were detected after intravenous injection of 6-OAP or vehicle in Kunming mice. L. A schematic representation of mechanisms of 6-OAP-induced mitosis arrest.