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. 2015 Sep 16;6(33):35004–35022. doi: 10.18632/oncotarget.5253

Figure 2. VPA acts as a radioprotector in normal cells and radiosensitizer in cancer cells.

Figure 2

Figure 2

A. Equal numbers of HT22 or GL261 cells were plated for colony formation assay after treating with PBS or 0.6 mM VPA for 7 days. Plates were stained with 1% methylene blue after 10 days and colonies were counted. Shown are bar graphs depicting the number of colonies for each treatment with SD from three experiments; *P < 0.05. B. HT22, Daoy, D54 and GL261 cell were treated with PBS (●) or 0.6 mM Valproic acid (■) for 7 days followed by irradiation with 0, 2, 4, 6 or 8 Gy and plated for clonogenic survival assay. Shown are the surviving fractions and the SD from three experiments; *P < 0.05. C. Equal numbers of HT22, Daoy, D54 and GL261 cells were plated in 96-well plates after treatment with PBS or 0.6 mM VPA for 7 days and then irradiated with 4Gy. After 96 h, the cell viability was determined using a colorimetric cell proliferation assay. Shown are the absorbances at 490 nm; *P < 0.05.