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. 2015 Sep 16;6(33):35023–35039. doi: 10.18632/oncotarget.5280

Figure 1. Evaluation of the in vitro cytotoxicity of CIK cells.

Figure 1

A. The proliferation ability of CNE2 and SUNE1 cells treated with CIK cells were analyzed by CCK8 assay. B. Colony formation assay for CNE2 and SUNE1 cells treated with CIK cells. C. NPC cells treated with CIK cells exhibited the reduced motility and invasion. The motility and invasion of CNE2 and SUNE1 cells were analyzed with an in vitro migration assay using a transwell chamber and an in vitro invasion assay using a matrigel-coated Boyden chamber, respectively. The migrated cells were plotted as the average number of cells per field of view from 3 different experiments, as described in the materials and methods section.