Figure 3. Activation of p53 by nutlin3a induces cell death in both proliferating and quiescent CD34⁺ progenitor cells from patients with BC CML cultured alone or co-cultured with MSCs.

CSFE stained CML cells were treated with nutlin3a for 48 h with or without MSC co-culture. Apoptotic cell death in CD34⁺ primitive CML cells was determined in proliferating (CSFE^dim) and quiescent (CSFE^bright) cells after cells were stained with CD34 antibody and annexin V in 7AAD negative cells. N3a, nutlin3a and cocx, co-culture. *, P < 0.05.