Figure 4. Protein (a) and mRNA (b-e) levels of genes involved in energy homeostasis in Drosophila melanogaster.

a. p-AMPK protein levels analyzed in whole fly homogenates following a 10 day treatment of male W¹¹¹⁸ Drosophila melanogaster with 10 mg/ml EGCG. b. spargel (srl), c. unpaired 2 (upd2), d. phosphoenolpyruvate carboxykinase (Pepck), and e. insulin-like peptide 5 (Ilp5) mRNA levels of genes involved in energy homeostasis measured in whole fly homogenates after a 10 day supplementation period of male W¹¹¹⁸ Drosophila melanogaster with 10 mg/ml EGCG. The data are expressed as the mean + SEM (n = 3, indicating extraction from 3 × 5 flies). * indicates significant differences in EGCG-treated flies compared to flies fed control medium for 10 days (p < 0.05, Student's t-test).