Table 1. Primer sequences (Drosophila melanogaster) used for real time PCR.

gene	forward primer (5′ → ‘3)	reverse primer (3′ → ‘5)	PCRproduct (bp)	annealing temperature (°C)
Ilp5	tgatggacatgctgagggtt	catgtggtgagattcggagc	128	57
Pepck	ccgccgagaaccttattgtg	agaatcaacatgtgctcggc	136	57
rpl32	ggcaagcttcaagatgacca	gttcgatcctaaccgatgt	198	58
spargel	ctcttggagtccgagatccgcaa	gggaccgcgagctgatggtt	90	64
upd2	atgatcctgagcgtcgtgat	cccgatgatgaggatgacga	131	59

Abbreviations: Ilp5 = insulin-like peptide 5; Pepck = phosphoenolpyruvate carboxykinase; rpl32 = ribosomal protein l32; upd2 = unpaired 2; bp = base pairs