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. 2015 Sep 22;6(31):30628–30639. doi: 10.18632/oncotarget.5790

Figure 1. The level of MTT assays in 0.2 Gy-irradiated neurons was increased compared to control cells.

Figure 1

A. E18 hippocampal neurons were irradiated with 0, 0.02, 0.2, or 2 Gy radiation on DIV 7. Cell survival was determined via MTT assays 1, 3 or 5 days after radiation treatment. Values are mean ± S.E.M from three independent experiments. (*:p < 0.05, paired Student's t test) B. Neurons were treated as in A.. Five days after radiation, cells were harvested for cell cycle analysis. C. E18 hippocampal neurons were irradiated with 0, 0.2, or 2 Gy radiation on DIV 7. Five days after radiation treatment, cells were fixed and stained by DAPI. Images were taken using Zeiss Observer Z1 microscope with 10x (NA/0.3) objective. Scale bar = 100 μm. D. Cell number was measured. Values are mean ± S.E.M from three independent experiments. (*:p < 0.05, paired Student's t test).