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. 2015 Sep 22;6(31):30628–30639. doi: 10.18632/oncotarget.5790

Figure 2. Radiation treatment did not have effects on mitochondrial membrane potential, ROS level, and mitochondrial DNA copy number.

Figure 2

A. E18 hippocampal neurons were irradiated with 0, 0.2, or 2 Gy radiation on DIV 7. Five days after radiation, neurons were collected and stained with JC-1 to measure mitochondrial membrane potential. B.-C. Neurons were treated as in A.. Five days after radiation, neurons were stained with MitoSOX Red and analyzed by BD Accuri C6 Flow Cytometer. Positive control on the left of B. showed increased MitoSOX Red for HUVEC cells treated with 40 μM carbonyl cyanide 4-(trifluoromethyoxy) phenylhydrazone (FCCP), an uncoupler of oxidative phosphorylation, for 30 min. Genomic DNA was extracted and mitochondrial DNA copy numbers were analyzed by Q-PCR. All quantified results are shown as mean ± S.E.M from three independent experiments.