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A. Expression of the Bcl-2 proteins was analyzed by western blotting, with GAPDH used as the internal control. B. Flow cytometry analysis of MMP by JC-1 staining. Cells were treated with 0, 4, 16, and 32 μM B5 for 48 h and stained with JC-1 for 20 min. Cells with MMP loss were gated. Data are presented as the mean ± SD of three independent experiments. *P < 0.5 and **P < 0.01.
