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A. CaSki and SiHa cells were treated with 16 μM B5 for 8h; ASK1-Trx complex was precipitated using Protein A beads and analyzed by western blotting with ASK1 and Trx antibodies. B. Activation of p38/JNK MAPKs. Cells were treated with 16 μM B5 for 0, 6, 12 and 24 h and subjected to western blotting analysis; GAPDH was used as the internal control.
