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. 2015 Aug 17;6(31):30993–31006. doi: 10.18632/oncotarget.5206

Figure 1. Ascl2 knockdown in CRC cell lines increased expression of goblet cell specific genes.

Figure 1

A. MUC2 mRNA levels in CRC cell lines (HT-29, shRNA-Ctr/HT-29, shRNA-Ascl2/HT-29, LS174T, shRNA-Ctr/LS174T and shRNA-Ascl2/LS174T cells) were quantitated by real-time PCR analysis twice, each in triplicate (n = 6). B. TFF3 mRNA levels in CRC cell lines were quantitated by real-time PCR analysis twice, each in triplicate (n = 6). C. and D. MUC2 protein expression in CRC cell lines and further densitometric analysis (n = 3). E. and F. Immunofluorescence staining of MUC2 protein in CRC cell lines.