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. 2015 Aug 17;6(31):30993–31006. doi: 10.18632/oncotarget.5206

Figure 2. Ascl2 knockdown in CRC cell lines increased CDX2 expression.

Figure 2

A. and B. Inhibition of Ascl2 in HT-29 and LS174T cells led to significant increase in CDX2 mRNA levels which were quantitated by real-time PCR analysis twice, each in triplicate (n = 6). C. and D. Inhibition of Ascl2 in HT-29 and LS174T cells led to significant increases in CDX2 protein levels which were detected by Western blotting and further densitometric analysis (n = 3). E. Immunofluorescence staining showed stronger CDX2 expression in shRNA-Ascl2/HT-29 cells compared with controls. F. Immunofluorescence staining showed stronger CDX2 expression in shRNA-Ascl2/LS174T cells compared with controls.