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. 2015 Aug 17;6(31):30993–31006. doi: 10.18632/oncotarget.5206

Figure 5. Identification of the cis-element that Ascl2 binds in the CDX2 promoter.

Figure 5

A. A schematic representation of the CDX2 promoter-Luc construct (−427/+417), which contains one E-box (CACCTG), and its mutant (CACCGG). B. shRNA-Ascl2/HT-29 and shRNA-Ctr/HT-29 cells were transfected with the CDX2 promoter-Luc construct (−427/+417) and its mutant to identify the sites of transcriptional regulation by Ascl2. C. shRNA-Ascl2/LS174T and shRNA-Ctr/LS174T cells were transfected with the CDX2 promoter-Luc construct (−427/+417) and its mutant to identify the sites of transcriptional regulation by Ascl2. The relative luciferase activity was determined using a dual-luciferase reporter assay system with a single sample luminometer. The data represented the mean±S.E. of three independent experiments (*: p < 0.05).