Table 1. The primer sequences used in the each CDX2 promoter fragment.
| pGL3-CDX2-promoter | Primer pairs | Length of product |
|---|---|---|
| −2167/+417 | F: 5′-TCTATCGATA GGTACC AGAGCCACGTCTTCAGG-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
2584 bp |
| −1681/+417 | F: 5′-TCTATCGATA GGTACC GGTCTCCCACCTCTTG-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
2098 bp |
| −1393/+417 | F: 5′-TCTATCGATA GGTACC GATGAAGGCGATGGTGAC-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
1810 bp |
| −1250/+417 | F: 5′-TCTATCGATA GGTACC TCGCCGCAGTGATCCT-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
1667 bp |
| −950/+417 | F: 5′-TCTATCGATA GGTACCAGTTGCCTTATCATCTCCT-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
1367 bp |
| −675/+417 | F: 5′-TCTATCGATA GGTACC TAACCACTAGCCTAACTTCT-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
1092 bp |
| −427/+417 | F: 5′-TCTATCGATA GGTACC ACAGGGCGGGAAGGAAT-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
844 bp |
| −272/+417 | F: 5′-TCTATCGATA GGTACC CAACCACTGCTCCTGTCT-3′ R: 5′-CTTAGATCGC AGATCT GCACGGAGCTAGGGTAC-3′ |
689 bp |
The underlined parts in the primers are KpnI and BglII adaptors.