Table 3. The primer sequences used in the ChIP experiment of CDX2 promoter.
ChIP | Primer pairs | Length of products |
---|---|---|
ChIP1 | Forward: 5′-GGCTAGTTCTGATCGCTTTC-3′ Reverse: 5′-TGTCACCATCGCCTTC-3′ |
181bp |
ChIP2 | Forward: 5′-GCTGGTTGTCACCTGTAAAA-3′ Reverse: 5′-CACTCCTGGAGACCTGC-3′ |
197bp |
ChIP3 | Forward: 5′-TACCTAGGTAAGCATTAGCA-3′ Reverse: 5′-GGCATGTGGTAGAAGTTAG-3′ |
196bp |
ChIP4 | Forward: 5′-AACGTTTAACAATAAATCGC-3′ Reverse: 5′-CAGCCCCAAACAACT-3′ |
158bp |
ChIP5 | Forward: 5′-TTCCCTGCAAATGCATAAAC-3′ Reverse: 5′-AGCTTCCTCCTTCCAACC-3′ |
158bp |
ChIP6 | Forward: 5′-TCGACGTCTCCAACCATT-3′ Reverse: 5′-CCTCCTTCCCACTAGGCT-3′ |
145bp |
ChIP for positive control (GAPDH) | Forward: 5′-TACTAGCGGTTTTACGGGCG-3′ Reverse: 5′-TCGAACAGGAGGAGCAGAGAGCGA-3′ |
166bp |