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. 2015 Sep 3;6(31):31151–31163. doi: 10.18632/oncotarget.5150

Figure 1. PCSCs are dependent on glutamine.

Figure 1

A. representative images of spheres derived from PANC-1 and SW1990 cells; subpopulations of PCSCs were evaluated via flow cytometry analysis. Scale bar: 100 μm. B. sphere formation assays were performed in the presence of different glutamine concentrations and sphere formation capacity was quantified by calculating the number of spheres per 5000 single cells. C. the effect of glutamine deprivation on secondary sphere formation was quantified by calculating the number of spheres formed from 100 single cells of passage one spheres. D. the effect of glutamine deprivation on the expression of indicated PCSC-related genes in spheres was measured by qRT-PCR. Error bars represent the mean ± S.D. of triplicate experiments. Statistical significance was calculated using the Student's t test or ANOVA. **p < 0.01; ***p < 0.001. ▼ replacing culture medium; ●, timing of evaluation.