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. 2015 Sep 3;6(31):31151–31163. doi: 10.18632/oncotarget.5150

Figure 4. Inhibition of non-canonical glutamine metabolism impairs sphere formation following continuous lose-dose IR treatment.

Figure 4

A. bright-field microscopy images showing features of shControl, shGLUD1, and shGLS1#2 pancreatic cancer cell-derived spheres following sphere-formation culture with 2 Gy IR treatment every second day. Scale bar: 200 μm. B. quantification of total number and average size of PANC-1 spheres in 4A after 10 days of sphere-formation culture. C. quantification of total number and average size of SW1990 spheres in 3A after 10 days of sphere-formation culture. Error bars represent the mean ± S.D. of triplicate experiments. Statistical significance was calculated using the Student's t test or ANOVA. **p < 0.01; ***p < 0.001.