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. 2015 Sep 5;6(31):31216–31232. doi: 10.18632/oncotarget.5157

Figure 2. Silencing effect of IKKα and IKKβ on cell cycle in human prostate cancer cells.

Figure 2

A. PC-3 and B. 22Rv1 cells were transfected with IKKα and IKKβ shRNA retroviral particles, a pool of viral particle containing 3 target specific constructs and one scrambled and one with negative shRNA that encode 19–25 nt (plus hairpin) designed to knockdown gene expression, selected under polybrene and used after 15–20 passage, stained with propidium iodide (50 mg/ml) and subjected to cell cycle analysis by flow cytometry. Percentage of cells in G0-G1, S and G2-M phase were calculated using Mod-fit computer software and are represented in the right side of the histograms. Knockdown of IKKα in both cell lines resulted in significant accumulation of cells in the G1 phase whereas IKKβ knockdown resulted in arrest in S phase of the cell cycle. Details are described in ‘materials and methods’ section.