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. 2015 Sep 4;6(31):31241–31254. doi: 10.18632/oncotarget.5160

Figure 3. Overexpression of SOX9 promotes cell proliferation and tumorigenesis of ESCC in vitro.

Figure 3

A. Western blotting analysis of SOX9 expression in ESCC cell lines. B. Effects of SOX9 on cell proliferation as analyzed by MTT assay. C. Representative micrographs (left) and quantification (right) of colonies formed by cells as determined by anchorage-independent growth assay. D. Representative micrographs (left) and quantification (right) of BrdU incorporation assay. E. Cell cycle progression as measured by flow cytometry. All experiments were performed using KYSE30 and KYSE140 cells stably overexpressing SOX9 or pMSCV-vector. Bars represent the mean ± SD of three independent experiments. *P < 0.05.