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. 2015 Sep 4;6(31):31241–31254. doi: 10.18632/oncotarget.5160

Figure 4. Silencing SOX9 suppresses the proliferation of ESCC cells.

Figure 4

A. Western blotting analysis of SOX9 expression in ESCC cell lines. B. Effects of SOX9 on cell proliferation as analyzed by MTT assay. C. Representative micrographs (left) and quantification (right) of colonies formed by cells as determined by anchorage-independent growth assay. D. Representative micrographs (left) and quantification (right) of the BrdU incorporation assay. E. Cell cycle progression as measured by flow cytometry. All experiments were performed using KYSE30 and KYSE140 cells in which SOX9 had been stably silenced or which expressed RNA interference (RNAi)-vector. Bars represent the mean ± SD of three independent experiments. *P < 0.05.