Table 1B. Infection of human dendritic cells with Tri-Ad5 vectors encoding transgenes can activate antigen-specific T-cell lines to produce IFN-γ.

Dendritic cells (DCs) infected with	Antigen-specific T-cell lines
	CEA(HLA-A2)	MUC1(HLA-A2)	MUC1(HLA-A24)	Brachyury(HLA-A2)
Tri-Ad5	480	236	763	496
Ad5 [E1, E2b]–null	<15.6	<15.6	<15.6	<15.6
Uninfected DCs	<15.6	<15.6	<15.6	<15.6
T cells only	<15.6	<15.6	<15.6	<15.6

Human DCs (6-day culture in IL-4 and GM-CSF) from an HLA-A2 and -A24 donor were infected with Tri-Ad5 vector at 2 × 10⁴ /well (24-well plate) in 0.5 ml of AIM-V. Tri-Ad5 vectors were used at 20,000 MOI for 1 hour and then 1.5 ml of AIM-V were added to each well. Infected DCs were incubated for 48 hours and then washed and used for stimulation of human antigen-specific T cells. Results are expressed in pg of IFN-γ per 1 × 10⁵ T cells/ml. Numbers in bold indicate a significant enhancement of IFN-γ secretion compared to corresponding wells with uninfected DCs.