Table 3. Infection of human DCs with Tri-Ad5 can generate brachyury-, MUC1- and CEA-specific CTLs that efficiently lyse tumor cells expressing all three antigens.

Antigen-specific T-cell lines	SW620 Brachyury+ MUC1+ CEA+ (HLA-A2+/A24+)	ASPC-1 Brachyury+ MUC1+ CEA+ (HLA-A1+/A26+)
T-brachyury	64.4 (3.6)	8.3 (2.7)
T-MUC1 (P93L)	28.5 (1.3)	2.0 (1.6)
T-MUC1 (C6A)	49.3 (3.3)	5.0 (1.8)
T-CEA	42.4 (3.7)	4.3 (1.9)

Human dendritic cells (DCs) were infected with Tri-Ad5 at 20, 000 MOI. Infected DCs were used to generate specific cytotoxic T lymphocytes (CTLs) using autologous peripheral blood monoclonal cells (PBMCs). Autologous DCs were used as antigen-presenting cells for three in vitro stimulations (IVS). Autologous peptide-pulsed B cells were used to re-stimulate antigen-specific CTLs for two additional IVS. The effector-to-target ratio used was 30:1; CTLs were used at IVS 5. Results are expressed in % specific lysis (SD).