Figure 6. Activation of MET signaling in SMS-CTR ERMS enhances tumor vascularization in vivo, whereas MET silencing in RH30 ARMS exerts the opposite effects.

A. Activated MET signaling increases number of capillaries in tumors formed by SMS-CTR ERMS and RH30 ARMS cells after subcutaneous implantation to NOD-SCID mice. Capillaries with erythrocytes were counted after staining of tumor sections for hematoxylin-eosin, n = 7–9. B. Representative images of the staining for CD31 demonstrates increased number of capillaries in TPR-MET SMS-CTR tumors in vivo. Number of CD31 positive capillaries was calculated in non-necrotic areas of tumor specimens, n = 4. *p < 0.05, **p < 0.01, ***p < 0.001. Data in graphs are represented as mean +/− SEM. Arrows indicate the capillaries.