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. 2015 Sep 30;6(31):31413–31427. doi: 10.18632/oncotarget.5105

Figure 1. CLIC1 protein is secreted by glioblastoma cells.

Figure 1

A. CLIC1 expression was analyzed by Western blot in cell lysates (left panel) and conditioned media (right panel) of several GBM cell lines. GAPDH was used as loading control. A representative immunoblot is shown. B. U87 MG cells were mock infected or infected with CLIC1 GFP fusion protein. Cells lysates and conditioned media were immunoblotted (Input), or immunoprecipitated against GFP-tag (IP anti-GFP) and then immunoblotted with CLIC1 antibody. A representative immunoblot is shown. C. U87 MG cells expressing Green Fluorescent Protein (GFP) were co-cultured with U87 MG cells expressing the FLAG-tagged isoform of CLIC1 for 24 hours. Cells were stained using anti-FLAG antibody (red). Arrowheads mark FLAG-tagged isoform of CLIC1 on U87 MG GFP cells. Scale bar, 20 μm.