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. 2015 Sep 30;6(31):31413–31427. doi: 10.18632/oncotarget.5105

Figure 3. CLIC1-containing EVs regulate the proliferative response of GBM cells.

Figure 3

A. Whole cell lysates (WCL) obtained from U87 MG control cells (NT), CLIC1 silenced cells (siCLIC1) and CLIC1 overexpressing cells (CLIC1 FLAG), as well as lysates of corresponding EVs, were analysed by Western blotting using antibodies against CLIC1, and the EV markers CD63 and tsg101. Vinculin was used as loading control. A representative immunoblot is shown. B. U87 MG cells were incubated with EVs (50 μg/ml) derived from U87 MG NT, U87 MG siCLIC1 and U87 MG CLIC1 FLAG cells, or PBS as control (veichle). Cell growth was measured 72, 96 and 120 hours after EV administration. Three independent experiments were performed; error bars represent standard error;