Table 2. Analysis of DNA and RNA: Primers in relation to assays used in the study.

	Size	Gene	Amplification Primers (5′ - 3′)	Sequencing Primers (5′ - 3′)	*Modified Sequence/corresponding unmodified sequence analyzed (5′ - 3′)
Q-PCR	346 bp	ITGA9-F	CCCCGCTGACTCGTTCTT
		ITGA9-R	TAGGATGTGGTCGGCTTC
	487 bp	WNT7A-F	GGGACTATGAACCGGAAAGC
		WNT7A-R	CGATGCCGTAGCGGATGT
	67 bp	GAPDH-F	AGCCACATCGCTCAGACAC
		GAPDH-R	GCCCAATACGACCAAATCC
BS-cloning sequencing	341 bp	ITGA9-F	TTGAGTGGGATTTGAGGATTTGTAT
		ITGA9-R	ACCTATCTCCCTACCTTTATCTCTCTTTC
	683 bp	WNT7A-F	TTTAGGTTGAGAAAGAGGTGGTTTA
		WNT7A-R	AACAAAACAACAAACCAAAACACTA
BS-pyrosequencing		ITGA9-S1-F	AGTGGGATTTGAGGATTTGT	AGGATTTGTATTT TTTGATAAG	TTTTTAGATGAYGTTGATGTTGTTGGTTYGGAG ATTATATTTYGGGAATTATTGGTGTAYGAGATTA AGTGATTAGAATATGGAG
		ITGA9-S1-R	biotin-CCCTCCATATTCTAATCACTTAAT		TTCCCAGATGACGTTGATGCTGCTGGTCCGGA GACCACACTTCGGGAATCACTGGTGTACGAG ACCAAGTGACTAGAATATGGAG
		ITGA9-S2-F	ATTGGGTGAAGGAGTAGAGGA	GAAGGAGTAGA GGATG	YGTAGGGTTAGAATTTGGAGTTTAAATTYGTT TTTTATTYGGGTTGTTTTGGATTTTATTGGTTG
		ITGA9-S2-R	biotin-CCCTCCCTATCTCACAACCAATAA		CGCAGGGCTAGAACCTGGAGTCTAAACTCGT CCTTCACCCGGGTTGCTCTGGACTTACTGGTTG
MSP	230 bp	ITGA9-F	GTTGTTGGTTCGGAGATTATATTTC
		ITGA9-R	AAAACAACCCGAATAAAAAACG
	123 bp	WNT7A-F	GTAGTTCGGCGTCGTTTTAC
		WNT7A-R	CGAAACCGTCTATCGATACG
	184 bp	β-ACTIN F	AAGTTAAGTTTTGTTTTTATTTTTTTT
		β -ACTIN R	CAATAATCTCCTTCTACATCCTATC

^*The cells with italic text show the unmodified sequences analyzed by bisulfite pyrosequencing.