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. 2015 Oct 2;6(31):31569–31592. doi: 10.18632/oncotarget.5035

Figure 9. Gene reporter assays and Western Blot in HEK 293T cells.

Figure 9

A. Dual-luciferase assays in transiently transfected cells with the pCZ-REN-P-LUC retroviral vectors containing the Renilla reporter under control of a constitutive promoter and the Firefly reporter under control of the gene specific promoter of the novel candidate genes. Presented are the average ratios of the fold changes of the reporter induction obtained in cells with ectopic over-expression of c-Myc compared to control cells with endogenous c-Myc expression. Error bars represent the standard deviation of at least three biological repeats. B. The same total protein extracts prepared for the luciferase assays was used to study the expression of c-Myc and Max proteins by Western blotting. β-actin served as loading control and house-keeping protein. The co-transfection of a c-Myc over-expression plasmid is also indicated (MIG-MYC).