Table 2. Validation of microarray data by RT-PCR.
| Gene | Method | Mean FC size of tumors | ||
|---|---|---|---|---|
| small | middle | large | ||
| Cdk4 | RT-PCR | 3.0 | 2.0 | 1.4 |
| Affymetrix | 2.7 | 2.1 | 2.9 | |
| Stk6 | RT-PCR | 6.6 | 3.1 | 4.1 |
| Affymetrix | 6.4 | 3.4 | 4.2 | |
| Nek6 | RT-PCR | 3.0 | 2.6 | 2.9 |
| Affymetrix | 3.1 | 3.2 | 3.5 | |
| Ccnb1 | RT-PCR | 9.5 | 4.0 | 5.4 |
| Affymetrix | 22.2 | 9.4 | 12.8 | |
| Cdc2a | RT-PCR | 13.3 | 6.5 | 8.3 |
| Affymetrix | 18.0 | 10.6 | 10.5 | |
| Prc1 | RT-PCR | 8.0 | 5.1 | 5.5 |
| Affymetrix | 6.0 | 3.0 | 4.2 | |
| Birc5 | RT-PCR | 10.1 | 4.8 | 4.9 |
| Affymetrix | 8.6 | 4.5 | 6.0 | |
| Hey1 | RT-PCR | A | −1.9 | A |
| Affymetrix | −4,9 | −3.7 | −5,1 | |
| Ddit3 | RT-PCR | −4.0 | −3.5 | −5.9 |
| Affymetrix | −2,3 | −3,5 | −6,2 | |
A - “Absent” - no gene expression was detected
The gene expression values in all samples were normalized to the beta-actin expression and used to calculate mean expression values for tumors of various sizes and control lung tissue extracts. The mean fold change was computed as a ratio between mean gene expression values for tumor sets and control non-transgenic lung as a baseline.