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. 2015 Sep 11;6(31):31702–31720. doi: 10.18632/oncotarget.5165

Figure 3. KDM4B promotes AR-mediated carcinogenesis in MFE-296 cells.

Figure 3

MFE-296 cells were transiently transfected with negative control (NC), AR (siAR) siRNA, KDM4B (siKDM4B) siRNA or KDM4B and AR siRNA together (siKDM4B+siAR). Cell proliferation was determined by MTT assay A. and colony formation assays B, C. Migrated and invasive MFE-296 cells on the lower surface of the Transwell filter were stained and photographed, 200×. The number of migrated and invasive cells is shown on the right. *P < 0.05, **P < 0.01, ***P < 0.005 compared with the NC group. D. KDM4B knockdown efficiency in shKDM4B group was confirmed by qRT-PCR. The tumor weight E. and tumor volumes F. and G. formed from nude mice injected subcutaneously with MFE-296 cells stably transfected with NC (MFE-296/NC) or shKDM4B (MFE-296/shKDM4B) were shown. H. Staining with hematoxylin and eosin (H&E) or immunohistochemical staining for KDM4B, AR, c-myc, and ki-67 in mouse tumor tissues (400×).