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. 2015 Sep 10;6(31):31721–31739. doi: 10.18632/oncotarget.5564

Figure 5. Hypoxia induced dedifferentiation employs both HIF-dependent and independent mechanisms.

Figure 5

A. Mammosphere formation efficiency in MCF-7 cells transfected with siHIF1α and/or siHIF2α, and cultured in normoxia or hypoxia for 3 days. B. Percentage of ALDH+ cells in T47D cells transfected with siHIF1α and/or siHIF2α, and cultured in normoxia or hypoxia. C. Percentage of CD44+CD24−/low cells from MDA-MB-468 cells transfected with siHIF1α and/or siHIF2α, and cultured in normoxia or hypoxia. Data are presented as mean ±SEM of 8 independent experiments. (D, E) HIF1α and HIF2α mRNA D. and protein E. expression in CSCs and non-CSCs from MDA-MB-468 cells cultured in normoxia or hypoxia. F. Percentage of CD44+CD24−/low MDA-MB-468 cells grown in normoxia after silencing all three PHDs individually or collectively or in hypoxia. G. Protein expression of HIF1α and HIF2α in MDA-MB-468 cells transfected with a control siRNA or a siRNA directed to PHD3 and cultured in normoxia or hypoxia. H., I. PHD1, PHD2 and PHD3 mRNA H. and protein I. expression levels in CSCs and non-CSCs sorted from MDA-MB-468 cells. B, D, F and H show means ±SD of three independent experiments.