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. 2015 Sep 10;6(31):31721–31739. doi: 10.18632/oncotarget.5564

Figure 7. Hypoxia-dependent CD24 downregulation is mediated through NFκB.

Figure 7

A. Analysis of CD24 mRNA stability by qPCR in the presence of actinomycin D during 16 h in normoxic or hypoxic conditions. B. CD24 mRNA expression levels in the absence or presence of C59 or DAPT, shown as fold change between hypoxia and normoxia. C. NFκB transcriptional activity in MDA-MB-231, MDA-MB-468 and BT549 cells transfected with a NFκB-dependent luciferase reporter. Data are represented as fold change activity in hypoxia versus normoxia. D. Percentage of CD44+CD24−/low in MDA-MB-468 cells, in the presence or absence of PS1145 (2 μM). Data are presented as fold change between hypoxia and normoxia. E. CD24 mRNA expression levels in the absence or presence of PS1145. F. Percentage of CD44+CD24−/low in MDA-MB-468 cells transfected with specific siRNA sequences against PHD3 in the presence or absence of PS1145. G. CD24 mRNA expression in MDA-MB-468 cells transfected with specific siRNA sequences against PHD3 in the presence or absence of PS1145. H. CD24 mRNA expression in BT549 cells transfected or not (untr) with a sh control (ctl) or shRelA/p65.