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. 2015 Sep 10;6(31):31780–31791. doi: 10.18632/oncotarget.5566

Figure 5. Ectopic over-expression of SOSTDC1 inhibits the expression of cyclinA2 and cyclin E2, and suppresses E2F transcriptional activity.

Figure 5

A. Western blotting analysis was performed to detect the cell cycle regulator cyclin A2, cyclin B1, cyclin D1, cyclin D2, cyclin D3, cyclin E1, cyclin E2, CDK2, CDK4, CDK6, p21Cip1, and p27Kip1 in indicated cells. α-tubulin was used as a loading control.B. qRT-PCR was conducted to detect the mRNA expression levels of cyclin A2 and cyclin E2 in indicated cells. GAPDH was used as a house keeping gene control. C. Ectopic expression of SOSTDC1 in the studied cells significantly inhibited the phosphorylation of pRb at Ser608 and Ser807 residues. α-tubulin served as the sample loading control. D. Over-expression of SOSTDC1 attenuates E2F transcriptional activity using E2F-luc reporter assay. For B. and D., results derived from three independent experiments are expressed as mean ± SD. *P < 0.05.