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. 2015 Sep 10;6(31):31805–31819. doi: 10.18632/oncotarget.5567

Figure 2. Anti-inflammatory effects of DMY in DNFB-induced DTH mice.

Figure 2

A. DMY dose-dependently inhibited acute ear edema of DTH-mice. B. DMY suppressed the nuclear translocation of NF-κB p65 in the ear tissues of DTH-mice. The bar chart shows the quantitation of Western blot results from three different animals within the same treatment groups. C. & D. DMY treatment did not affect the weight of the spleen, thymus or whole body in DTH-mice. Five groups of mice were challenged with DNFB to develop DTH. The DTH mice were then treated with or without the reference drug DEX or the indicated dosage of DMY for 4 days. The acute ear edema tissue, spleen and thymus of DTH mice were dissected and weighed at the end of drug treatment, while the body weight of animals was assessed daily. The nuclear protein lysates of the ear tissues of DTH mice were prepared for Western blotting analysis using the antibody against p65. Data are expressed as means ± SEM (n = 9-12), *P < 0.05, **P < 0.01, ***P < 0.001 compared to vehicle-treated mice.