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. 2015 Sep 10;6(31):31927–31943. doi: 10.18632/oncotarget.5578

Figure 5. Icaritin treatment downregulates IL-6Rs expression and IL-6 signaling.

Figure 5

PLC/PRF/5 (A) and Huh7 (B) cells were treated with the indicated concentrations of Icaritin for 24 h. Total cell lysates were prepared for western blot analysis using gp130, gp80 and GAPDH antibodies. (C, D). Western blots analysis with Oct4, Bmi-1 and GAPDH antibodies. (E). Cells transfected with the vector control or the Stat3-C expression plasmid were treated with DMSO or Icaritin (4 μM) for 24 h. Western blots were performed with Bmi-1, Oct4, and GAPDH antibodies. ICT, Icaritin.